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Developmental Biology

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JoVE Journal Developmental Biology
Voorbereiding van kleine RNA-bibliotheken voor sequencing van vroege muisembryo's
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Voorbereiding van kleine RNA-bibliotheken voor sequencing van vroege muisembryo's

Article DOI: 10.3791/61086-v • 08:37 min • October 9th, 2020
October 9th, 2020 •
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1Development, Disease Models & Therapeutics Graduate Program, Department of Molecular and Cellular Biology, Department of Neuroscience, Center for Cell and Gene Therapy, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine

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We beschrijven een techniek voor het profileren van microRNAs in vroege muisembryo's. Dit protocol overwint de uitdaging van lage celinput en kleine RNA-verrijking. Deze test kan worden gebruikt om veranderingen in miRNA expressie na verloop van tijd te analyseren in verschillende celvormen van het vroege muisembryo.

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Ontwikkelingsbiologie neurale kam microRNA klein-RNA sequencing ontwikkelingstiming post-transcriptional regulatie muis
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