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Journal / Immunology and Infection / Driedimensionale beeldvorming van de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Driedimensionale beeldvorming van de wervellymfefelietische vasculatuur en drainage met behulp van iDISCO+ en Light Sheet Fluorescentie Microscopie
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Driedimensionale beeldvorming van de wervellymfefelietische vasculatuur en drainage met behulp van iDISCO+ en Light Sheet Fluorescentie Microscopie

Article DOI: 10.3791/61099-v • 10:05 min • May 22nd, 2020
May 22nd, 2020 •
*1, *1,2, 1,3
1Université Pierre et Marie Curie Paris, Sorbonne Université, Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 3Department of Neurology, Yale University School of Medicine
* These authors contributed equally

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Een protocol wordt gepresenteerd combineren weefsel clearing met licht blad fluorescentie microscopie (LSFM) om driedimensionale en cellulaire resolutie beelden van de lymfevaten en lymfeklieren (LN' s) het verzamelen van de cerebrospinale vloeistof (CSF) en spinale epidurale vloeistof te verkrijgen.

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Immunologie en infectie Probleem 159 meningeale en wervellymfefeliet lymfeklieren drainage iDISCO+ lichtblad fluorescentiemicroscoop centraal zenuwstelsel
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