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JoVE Journal Neuroscience
I Utero Electroporation af Multiaddressable Genome-Integration Color (MAGIC) Markører til individualisere Cortical Mouse Astrocytes
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I Utero Electroporation af Multiaddressable Genome-Integration Color (MAGIC) Markører til individualisere Cortical Mouse Astrocytes

Article DOI: 10.3791/61110-v • 06:47 min • May 21st, 2020
May 21st, 2020 •
*1, *2, 1, 3, 4, 2, 1, 4, 3, 2, 1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
* These authors contributed equally

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Astrocytter flise hjernebarken ensartet, hvilket gør analysen af deres komplekse morfologi udfordrende på cellulært niveau. Protokollen her bruger flerfarvet mærkning baseret på in utero elektroporation til at fremhæve kortikale astrocytter og analysere deres volumen og morfologi med en brugervenlig billedanalysepipeline.

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Neurovidenskab Problem 159 hjernebark astrocytter udvikling cellevolumen morfologi transgener elektroporation genoverførselsteknikker mikroskopi
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