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JoVE Journal Neuroscience
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Em Utero Eletroporation de Marcadores de Cor Multiaddressable (MAGIC) para Individualizar Astrócitos de Ratos Cortical

Article DOI: 10.3791/61110-v • 06:47 min • May 21st, 2020
May 21st, 2020 •
*1, *2, 1, 3, 4, 2, 1, 4, 3, 2, 1
1Université de Montpellier, INSERM, Institut des Neurosciences de Montpellier, 2Sorbonne Université, INSERM, CNRS, Institut de la Vision, 3Université Paris-Saclay, CEA, CNRS, MIRCen, Laboratoire des Maladies Neurodégénératives, 4Laboratory for Optics and Biosciences, Ecole polytechnique, CNRS, INSERM, IP Paris
* These authors contributed equally

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Os astrócitos ladrilho o córtex cerebral uniformemente, tornando a análise de sua morfologia complexa desafiadora a nível celular. O protocolo aqui fornecido usa rotulagem multicolorida baseada na eletroporação uterónica para destacar os astrócitos corticais e analisar seu volume e morfologia com um pipeline de análise de imagem fácil de usar.

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Neurociência Questão 159 córtex cerebral astrócitos desenvolvimento volume celular morfologia transgenes eletroporação técnicas de transferência genética microscopia
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