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JoVE Journal
Neuroscience

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研究原发性胚胎小鼠中脑多巴胺神经元中预制纤维诱导β-合成素积累
 
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研究原发性胚胎小鼠中脑多巴胺神经元中预制纤维诱导β-合成素积累

Article DOI: 10.3791/61118-v 10:03 min August 16th, 2020
August 16th, 2020

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Summary

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Click here for the English version.

在这里,我们提出了一个详细的协议,研究神经元+-合成素积累在原发性小鼠多巴胺神经元。神经元中的磷酸化β-合成素聚合体通过预制的β-合成素纤维诱导。免疫荧光标记细胞的自动成像和无偏图像分析使这个强大的协议适用于抑制β-合成素积累的药物的中高通量筛选。

Tags

神经科学,第162期,原发性胚胎多巴胺神经元,α-核素,预制纤维,Lewy身体,高含量图像分析,帕金森病,三核素病
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