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Journal / Neuroscience / Undersøgelse Præ-dannet Fibril Induceret…
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Neuroscience

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JoVE Journal Neuroscience
Undersøgelse Præ-dannet Fibril Induceret α-Synuclein ophobning i primær embryonale mus Midbrain dopamin neuroner
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Undersøgelse Præ-dannet Fibril Induceret α-Synuclein ophobning i primær embryonale mus Midbrain dopamin neuroner

Article DOI: 10.3791/61118-v • 10:03 min • August 16th, 2020
August 16th, 2020 •
1, 1, 2, 1,3, 1
1Institute of Biotechnology, HiLIFE, University of Helsinki, 2Neuroscience Center, HiLIFE, University of Helsinki, 3Department of Brain Biochemistry, Maj Institute of Pharmacology, Polish Academy of Sciences

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Her præsenterer vi en detaljeret protokol til undersøgelse af neuronal α-synucleinakkumulering i primære muse dopaminneuroner. Fosforylerede α-synucleinaggregater i neuroner induceres med præformede α-synuclein fibrils. Automatiseret billeddannelse af immunfluorescent mærket celler og upartisk billedanalyse gør denne robuste protokol egnet til medium-til-høj gennemløb screening af lægemidler, der hæmmer α-synuclein akkumulering.

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Neurovidenskab primær embryonal dopamin neuroner α-synuclein præ-formede fibriller Lewy krop højt indhold billedanalyse Parkinsons sygdom synucleinopati
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