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Evaluering af oxidativ stress i biologiske prøver ved hjælp af analysen af thiobarbitursyrereaktive stoffer
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Evaluering af oxidativ stress i biologiske prøver ved hjælp af analysen af thiobarbitursyrereaktive stoffer

Article DOI: 10.3791/61122-v • 06:19 min • May 12th, 2020
May 12th, 2020 •
1,
1School of Molecular Sciences, The Biodesign Institute - Center for Personalized Diagnostics, Arizona State University

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Målet med analysen af reaktive stoffer af thiobarbitursyre er at vurdere oxidativ stress i biologiske prøver ved at måle produktionen af lipidperoxideringsprodukter, primært malondialdehyd, ved hjælp af synlig bølgelængdespektrofotometri ved 532 nm. Den metode, der er beskrevet her, kan anvendes på humane serum, cellelyater og lipoproteiner med lav densitet.

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Kemi Udgave 159 thiobarbituric syre reaktive stoffer TBARS oxidation lipidperoxidation malondialdehyd MDA thiobarbitursyre TBA oxidativ stress
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