JoVE Journal
Developmental Biology
Developmental Biology
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从鼠心心室自由壁分离内膜细胞和冠状内皮细胞
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Article
DOI:
10.3791/61126-v
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08:22 min
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April 15th, 2020
从鼠心心室自由壁分离内膜细胞和冠状内皮细胞
April 15th, 2020
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1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
我们提出了一种方案,通过消化缓冲液中的连续组织消化、循环离心循环的细胞采集以及使用抗大鼠CD31微珠进行细胞纯化,从大鼠心脏分离内皮和冠状内皮细胞。