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Journal / Developmental Biology / Isolering av endokardiella och koronar…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering av endokardiella och koronar endotelceller från Ventrikulärt Fria väggen i Råtthjärtat
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Isolering av endokardiella och koronar endotelceller från Ventrikulärt Fria väggen i Råtthjärtat

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Vi presenterar ett protokoll för isolering av endokardiella och födans endotel celler från råtta hjärtan genom sekventiell vävnad matsmältningen i en matsmältningen buffert, cell insamling från återkommande centrifug cykler och cell rening med anti-råtta CD31 microbeads.

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Utvecklingsbiologi utgåva 158 endokardi koronaretittel isolering hjärta råtta CD31
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