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Journal / Genetics / 使用纳米流体技术的单体和散装 C. 电子样品 中的高通量定量…
JoVE Journal
Genetics

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JoVE Journal Genetics
使用纳米流体技术的单体和散装 C. 电子样品 中的高通量定量 RT-PCR
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Journal (Genetics)
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
Journal (Genetics)
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
 
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使用纳米流体技术的单体和散装 C. 电子样品 中的高通量定量 RT-PCR

Article DOI: 10.3791/61132-v • 08:19 min • May 28th, 2020
May 28th, 2020 •
*1, *2,3,5, 1, 1, 1, 2,3,4, 1, 1
1Babraham Institute, 2Gurdon Institute, University of Cambridge, 3Department of Genetics, University of Cambridge, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease, The Rockefeller University
* These authors contributed equally

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Summary
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本文描述了一个高通量协议，用于快速、可靠地确定单个或批量 C.elegans 样本中的基因表达水平。此协议不需要 RNA 隔离，并且直接从样品中生成 cDNA。它可以与高通量多路复用纳米流体实时 qPCR 平台一起使用。

Tags

遗传学， 问题 159， RT-qPCR， 单蠕虫， C. elegans， 散装样品， 蠕虫到 CT， 线虫， 高通量， 纳米流体， 多路复用
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