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Journal / Genetics / Kvantitative RT-PCR'er med høj gennemløb i…
JoVE Journal
Genetics

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JoVE Journal Genetics
Kvantitative RT-PCR'er med høj gennemløb i enkelt- og bulk-C. elegans-prøver ved hjælp af nanofluidisk teknologi
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Kvantitative RT-PCR'er med høj gennemløb i enkelt- og bulk-C. elegans-prøver ved hjælp af nanofluidisk teknologi

Article DOI: 10.3791/61132-v • 08:19 min • May 28th, 2020
May 28th, 2020 •
*1, *2,3,5, 1, 1, 1, 2,3,4, 1, 1
1Babraham Institute, 2Gurdon Institute, University of Cambridge, 3Department of Genetics, University of Cambridge, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease, The Rockefeller University
* These authors contributed equally

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I denne artikel beskrives en protokol med høj overførselshastighed til hurtig og pålidelig bestemmelse af genekspressionsniveauer i enkelt- eller bulk-C. elegans-prøver. Denne protokol kræver ikke RNA-isolering og producerer cDNA direkte fra prøver. Det kan bruges sammen med high-throughput multiplexed nanofluidic real-time qPCR platforme.

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Genetik RT-qPCR single-orm C. elegans bulk prøver orm-til-CT nematoder høj-throughput nanofluidic multiplex
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