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Journal / Genetics / Kwantitatieve RT-PCR met hoge doorvoer in enkele…
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Genetics

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JoVE Journal Genetics
Kwantitatieve RT-PCR met hoge doorvoer in enkele en bulk C. elegans-monsters met behulp van nanofluïdische technologie
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Kwantitatieve RT-PCR met hoge doorvoer in enkele en bulk C. elegans-monsters met behulp van nanofluïdische technologie

Article DOI: 10.3791/61132-v • 08:19 min • May 28th, 2020
May 28th, 2020 •
*1, *2,3,5, 1, 1, 1, 2,3,4, 1, 1
1Babraham Institute, 2Gurdon Institute, University of Cambridge, 3Department of Genetics, University of Cambridge, 4Wellcome Trust Genome Campus, Wellcome Trust Sanger Institute, 5Laboratory of Virology and Infectious Disease, The Rockefeller University
* These authors contributed equally

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In dit artikel wordt een protocol met hoge doorvoer beschreven voor een snelle en betrouwbare bepaling van de genexpressieniveaus in enkele of bulk C. elegansmonsters. Dit protocol vereist geen RNA-isolatie en produceert cDNA rechtstreeks uit monsters. Het kan worden gebruikt samen met high-throughput multiplexed nanofluidic real-time qPCR platforms.

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Genetica RT-qPCR single-worm C. elegans,bulk samples worm-to-CT nematoden high-throughput nanofluidic multiplex
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