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Journal / Biology / Worm-align and Worm_CP, Two Open-Source Pipelines …
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Biology

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JoVE Journal Biology
Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans
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Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

Article DOI: 10.3791/61136-v • 11:16 min • May 28th, 2020
May 28th, 2020 •
1, 1, 1, 1, 1
1Babraham Institute

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Worm-align/Worm_CP ist ein einfacher FIJI/CellProfiler-Workflow, der verwendet werden kann, um Caenorhabditis elegans-Proben zu begradigen und auszurichten und bildbasierte Tests zu bewerten, ohne dass vorherige Trainingsschritte erforderlich sind. Wir haben Worm-align/Worm_CP auf die Quantifizierung der hitzeschockinduzierten Expression bei lebenden Tieren oder Lipidtröpfchen in festen Proben angewendet.

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Biologie Ausgabe 159 C. elegans Fluoreszenz Quantifizierung Wurmbegradigung CellProfiler FIJI Bild J
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