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JoVE Journal
Biology

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Massespektrometri analyse til at identificere allestedsnærværendelation af EYFP-tagged CENP-A (EYFP-CENP-A)
 
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Massespektrometri analyse til at identificere allestedsnærværendelation af EYFP-tagged CENP-A (EYFP-CENP-A)

Article DOI: 10.3791/61138-v 09:02 min June 10th, 2020
June 10th, 2020

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Summary

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CENP-A allestedsnærværendelation er et vigtigt krav til CENP-A deposition på centromere, arvet gennem dimerization mellem celledeling, og uundværlig for celle levedygtighed. Her beskriver vi massespektrometrianalyse for at identificere allestedsnærværendelation af EYFP-mærket CENP-A (EYFP-CENP-A) protein.

Tags

Biologi centromere kinetochore mitose CENP-A post-translationel modifikation PTM allestedsnærværendelation massespektrometri analyse
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