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여기에서는 piggyBac계 공여 플라스미드와 Cas9 nickase 돌연변이체를 이용한 인간 만능줄기세포에서 원활한 유전자 편집을 위한 상세한 방법을 설명한다. 인간 배아 줄기 세포 (hESCs)에서 간세포 핵 인자 4 알파 (HNF4α) 유전자좌의 엑손 8에 2 점 돌연변이가 도입되었다.