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Journal / Genetics / Introduktion av punktmutationer i mänskliga…
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Genetics

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JoVE Journal Genetics
Introduktion av punktmutationer i mänskliga pluripotenta stamceller med sömlös genomredigering
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Introduktion av punktmutationer i mänskliga pluripotenta stamceller med sömlös genomredigering

Article DOI: 10.3791/61152-v • 09:03 min • May 10th, 2020
May 10th, 2020 •
1, 1, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh

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Här beskriver vi en detaljerad metod för sömlös genredigering i mänskliga pluripotenta stamceller med hjälp av en piggyBac-baserad donatorplasmid och Cas9 nickase-mutanten. Tvåpunktsmutationer infördes i exon 8 av hepatocytkärnfaktorn 4 alfa (HNF4α) locus i mänskliga embryonala stamceller (hESC).

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Genetik nummer 159 genomredigering CRISPR sömlösa pluripotenta stamceller piggyBac knock-in
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