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Journal / Bioengineering / Fremstilling af Zero Mode Waveguides for høj…
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JoVE Journal Bioengineering
Fremstilling af Zero Mode Waveguides for høj koncentration enkelt molekyle mikroskopi
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Fremstilling af Zero Mode Waveguides for høj koncentration enkelt molekyle mikroskopi

Article DOI: 10.3791/61154-v • 08:01 min • May 12th, 2020
May 12th, 2020 •
1, 1, 1, 2, 2, 1
1Pennsylvania Muscle Institute, Perelman School of Medicine, University of Pennsylvania, 2Department of Physics, West Chester University

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Beskrevet her er en nanosfære litografi metode til parallel fremstilling af nul mode waveguides, som er arrays af nanoapertures i en metal-klædt glas mikroskopi coverslip for enkelt molekyle billeddannelse på nano-til mikromolar koncentrationer af fluorophores. Metoden udnytter kolloid krystal selvmontering til at oprette en waveguide skabelon.

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Bioengineering Problem 159 nul-tilstand waveguides nano-blænde enkelt molekyle fluorescens nanosfære litografi kolloid krystal selvmontering
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