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Journal / Biology / İmmünfluoresan siyon ile Primer Silya'nın Basit…
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İmmünfluoresan siyon ile Primer Silya'nın Basit Tespiti
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İmmünfluoresan siyon ile Primer Silya'nın Basit Tespiti

Article DOI: 10.3791/61155-v • 08:07 min • May 15th, 2020
May 15th, 2020 •
1, 2, 3, 4, 1, 1
1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

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Primer silia centriole ile ilişkili hücre dışı yapılardır. İmmünfloresan boyama ile primer silia tespiti son derece yüksek kaliteli görüntülerle sonuçlanan nispeten basit bir işlemdir. Bu protokolde primer silya yı ifade eden fibroblastlar sabit, immünolekeli ve floresan veya konfokal mikroskopta görüntülendi.

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Biyoloji Sayı 159 primer silia immünofloresan fibroblastlar asetilalfa tubulin gama tubulin mikroskopi tüp bebek ekimi serum açlık ışınlama doksorubisin taxol.
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