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Semplice rilevamento delle ciglia primarie per immunofluorescenza
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Semplice rilevamento delle ciglia primarie per immunofluorescenza

Article DOI: 10.3791/61155-v • 08:07 min • May 15th, 2020
May 15th, 2020 •
1, 2, 3, 4, 1, 1
1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove, University of Defence, 2Department of Clinical Biochemistry and Diagnostics, University Hospital, 3Department of Oncology, Thomayer Hospital, Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine, Charles University

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Le ciglia primarie sono strutture extracellulari associate al centriole. Il rilevamento delle ciglia primarie mediante colorazione immunofluorescente è una procedura relativamente semplice che si traduce in immagini di altissima qualità. In questo protocollo, i fibroblasti che esprimevano ciglia primarie sono stati fissati, immunizzati e immagini in un microscopio fluorescente o confocale.

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