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JoVE Journal
Immunology and Infection

This content is Open Access.

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions
 

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

Article doi: 10.3791/61165
May 16th, 2020

Summary May 16th, 2020

This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit plating.

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