Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection

This content is Open Access.

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions
 

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

Article DOI: 10.3791/61165-v 09:42 min May 16th, 2020
May 16th, 2020

Chapters

Summary

This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit plating.

Transcript

Tags

Zebrafish Larvae Aspergillus Infection Host-pathogen Interactions Immune Responses Larval Zebrafish Model Therapeutic Targets Invasive Aspergillosis Microinjections Pressure Injector Vac Pressure Unit Foot Switch Micropipette Holder Micro Manipulator Magnetic Stand And Plate Compressed Air Valve Microinjector PBS Spore Suspension Phenol Red Aspergillus Fumigatus
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter