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Isolering af humane ventrikulære kardiomyocytter fra Vibratome-Cut Myocardial Slices
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Isolering af humane ventrikulære kardiomyocytter fra Vibratome-Cut Myocardial Slices

Article DOI: 10.3791/61167-v • 11:19 min • May 10th, 2020
May 10th, 2020 •
1, 1,2, 1,2
1Institute of Cellular and Molecular Physiology, Friedrich-Alexander University Erlangen-Nürnberg, 2Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg

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Præsenteret er en protokol for isolering af menneskelige og animalske ventrikulære kardiomyocytter fra vibratom-cut myokardie skiver. Høje udbytter af calciumtolerante celler (op til 200 celler/mg) kan opnås fra små mængder væv (<50 mg). Protokollen gælder for myokardiet, der udsættes for kold iskæmi i op til 36 timer.

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Biologi human hjertesvigt myokardie skiver cardiomyocyte isolation vibratome kontraktile myocytter calcium billeddannelse hjerte excitation-sammentrækning kobling
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