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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikation og karakterisering af immunogeneRNA-arter i HDM-allergener, der modulerer eosinofil lungebetændelse
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Identifikation og karakterisering af immunogeneRNA-arter i HDM-allergener, der modulerer eosinofil lungebetændelse

Article DOI: 10.3791/61183-v • 08:44 min • May 30th, 2020
May 30th, 2020 •
1,2, 1,3, 1
1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

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Miljømæssige allergener såsom husstøvmider (HDM) indeholder ofte mikrobielle stoffer, der aktiverer medfødte immunreaktioner for at regulere allergisk inflammation. Den protokol, der præsenteres her, viser identifikationen af dsRNA-arter i HDM-allergener og karakterisering af deres immunogene aktiviteter i modulerende eosinofil lungebetændelse.

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Immunologi og infektion BAL bronchoalveolar lavage dsRNA FACS fluorescens-aktiveret cellesortering HDM hus støvmider ISGs interferon stimuleret gener PAMP patogen-associeret molekylærmønster RT-qPCR
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