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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifizierung und Charakterisierung immunogener RNA-Arten in HDM-Allergenen, die eosinophile Lungenentzündungen modulieren
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Identifizierung und Charakterisierung immunogener RNA-Arten in HDM-Allergenen, die eosinophile Lungenentzündungen modulieren

Article DOI: 10.3791/61183-v • 08:44 min • May 30th, 2020
May 30th, 2020 •
1,2, 1,3, 1
1Department of Microbiology, Immunology and Molecular Genetics, Long School of Medicine, University of Texas Health San Antonio, 2Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, 3Department of Otolaryngology-Head and Neck Surgery, Xiangya Hospital, Central South University

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Umweltallergene wie Hausstaubmilben (HDM) enthalten oft mikrobielle Substanzen, die angeborene Immunreaktionen aktivieren, um allergische Entzündungen zu regulieren. Das hier vorgestellte Protokoll zeigt die Identifizierung von dsRNA-Arten in HDM-Allergenen und die Charakterisierung ihrer immunogenen Aktivitäten bei der Modulation eosinophiler Lungenentzündungen.

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Immunologie und Infektion Ausgabe 159 BAL Bronchoalveolar-Lavage dsRNA FACS Fluoreszenz-aktivierte Zellsortierung HDM Hausstaubmilben ISGs Interferon-stimulierte Gene PAMP pathogenassoziiertes molekulares Muster RT-qPCR
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