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In Vivo Quantificação do Volume de Negócios de Proteínas no Envelhecimento C. Elegans usando Dendra2 Fotoconvertível
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In Vivo Quantificação do Volume de Negócios de Proteínas no Envelhecimento C. Elegans usando Dendra2 Fotoconvertível

Article DOI: 10.3791/61196-v • 09:45 min • June 13th, 2020
June 13th, 2020 •
1,2, 1,3
1Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund, 2NeuroCure Cluster of Excellence, Charité - Universitätsmedizin Berlin, 3Department of Biology and Chemistry, University of Bremen

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Apresentado aqui é um protocolo para monitorar a degradação da proteína huntingtin fundida ao fluorohore fotoconvertível Dendra2.

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Biologia Edição 160 C. elegans,huntingtin rede de proteostase degradação Dendra2 fotoconversão microscopia confocal Fiji/ImageJ
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