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Biochemistry

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JoVE Journal Biochemistry
Evaluación de la oxidación celular utilizando una proteína fluorescente verde sensible al redox específica del compartimento subcelular
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Evaluación de la oxidación celular utilizando una proteína fluorescente verde sensible al redox específica del compartimento subcelular

Article DOI: 10.3791/61229-v • 06:10 min • June 18th, 2020
June 18th, 2020 •
1,2, 1, 1, 1
1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

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Este protocolo describe la evaluación del estado redox subcelular específico del compartimento dentro de la célula. Una sonda fluorescente sensible al redox permite un análisis ratiométrico conveniente en las células intactas.

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Bioquímica Número 160 proteína fluorescente verde sensible al redox (roGFP) roGFP citosólico relación cisteína/cistina imágenes de células vivas citometría de flujo oxidación/reducción
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