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Parallell høy gjennomstrømning enkelt molekyl kinetisk analyse for stedsspesifikk DNA cleavage
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Parallell høy gjennomstrømning enkelt molekyl kinetisk analyse for stedsspesifikk DNA cleavage

Article DOI: 10.3791/61236-v • 06:51 min • May 6th, 2020
May 6th, 2020 •
1, 1, 2
1Department of Biology, Emmanuel College, 2Department of Chemistry and Physics, Emmanuel College

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En svært parallell metode for måling av stedsspesifikk spaltning av DNA på enkeltmolekylnivå er beskrevet. Denne protokollen viser teknikken ved hjelp av restriksjonen endonuclease NdeI. Metoden kan enkelt endres for å studere enhver prosess som resulterer i stedsspesifikk DNA-spalting.

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Biologi utgave 159 DNA-tilknytning enkeltmolekyl DNA-spalting protein-DNA-interaksjoner begrensningsdonukleær stedsspesifikk DNA-binding
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