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JoVE Journal Neuroscience
Medición de interacciones transcelulares a través de la agregación de proteínas en un sistema celular heterologoso
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Medición de interacciones transcelulares a través de la agregación de proteínas en un sistema celular heterologoso

Article DOI: 10.3791/61237-v • 04:47 min • May 22nd, 2020
May 22nd, 2020 •
1, 1, 1, 1
1Department of Pharmacology, University of Colorado Denver School of Medicine

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Aquí, presentamos un protocolo optimizado para medir rápida y semicuantitativamente las interacciones ligando-receptor en trans en un sistema celular heterologoso utilizando microscopía de fluorescencia.

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Neurociencia Número 159 agregación ligandos sinápticos HEK293T interacciones con proteínas trans Neurexin LRRTM Adhesión celular
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