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Cultivo de pericitos capilares cerebrales para mediciones de calcio citosólico y estudios de imágenes de calcio
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Cultivo de pericitos capilares cerebrales para mediciones de calcio citosólico y estudios de imágenes de calcio

Article DOI: 10.3791/61253-v • 09:35 min • May 27th, 2020
May 27th, 2020 •
1, 1, 1
1Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen

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Los pericitos capilares cerebrales son actores esenciales en la regulación de las propiedades de la barrera hematoencefálica y el flujo sanguíneo. Este protocolo describe cómo los pericitos capilares cerebrales pueden ser aislados, cultivados, caracterizados con respecto al tipo de célula y aplicados para investigaciones de señalización de calcio intracelular con sondas fluorescentes.

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Neurociencia Número 159 Pericito ATP Ca2+-señalización Fura-2 AM Cal-520 AM cultivo celular lector de placas microscopía de escaneo láser confocal
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