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JoVE Journal Neuroscience
Kultur af Brain Kapillære Pericytes for cytosolic calcium målinger og calcium Imaging Undersøgelser
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Kultur af Brain Kapillære Pericytes for cytosolic calcium målinger og calcium Imaging Undersøgelser

Article DOI: 10.3791/61253-v • 09:35 min • May 27th, 2020
May 27th, 2020 •
1, 1, 1
1Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen

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Hjernen kapillær pericytter er væsentlige aktører i reguleringen af blod - hjerne barrieren egenskaber og blodgennemstrømning. Denne protokol beskriver, hvordan hjernen kapillær pericytter kan isoleres, kulturperler, karakteriseret med hensyn til celletype og anvendes til undersøgelser af intracellulære calcium signalering med fluorescerende sonder.

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Neurovidenskab Pericyte ATP Ca2 +-signalering Fura-2 AM Cal-520 AM celle kultur pladelæser konfokal laserscanning mikroskopi
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