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Journal / Cancer Research / 在结肠直肠癌细胞的三维球体培养物中使用无细胞益生菌超常剂评估细胞死亡…
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Cancer Research

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JoVE Journal Cancer Research
在结肠直肠癌细胞的三维球体培养物中使用无细胞益生菌超常剂评估细胞死亡
Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
Journal (Cancer Research)
Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
Journal (Cancer Research)
A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
 
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在结肠直肠癌细胞的三维球体培养物中使用无细胞益生菌超常剂评估细胞死亡

Article DOI: 10.3791/61285-v • 06:07 min • June 13th, 2020
June 13th, 2020 •
*1,2, *3, 1,4,5, 1, 1
1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST)
* These authors contributed equally

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Summary
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这里介绍了了解 乳酸杆菌 无细胞超高分子（LCFS）的抗癌作用的方法。结肠直肠癌细胞系显示在3D培养物中用LCFS治疗时细胞死亡。生成球体的过程可以根据脚手架进行优化，并且所呈现的分析方法可用于评估所涉及的信号通路。

Tags

癌症研究， 第 160 期， 益生菌， 乳酸杆菌发酵， 结肠直肠癌， 球状体， 3D 培养， 乳酸杆菌无细胞超高分子 （LCFS）
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