Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / X-Ray krystallografi at studere oligomeric stat…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
X-Ray krystallografi at studere oligomeric stat Overgang af Thermotoga maritima M42 Aminopeptidase TmPep1050
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
Journal (Biochemistry)
Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Journal (Biochemistry)
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
 
Click here for the English version

X-Ray krystallografi at studere oligomeric stat Overgang af Thermotoga maritima M42 Aminopeptidase TmPep1050

Article DOI: 10.3791/61288-v • 11:27 min • May 13th, 2020
May 13th, 2020 •
1,2, 2, 1, 1
1Laboratory of Microbiology, Department of Molecular Biology, Université Libre de Bruxelles, 2Labiris Institut de Recherche

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol er udviklet til at studere dimer-dodecamer overgang af TmPep1050, en M42 aminopeptidase, på det strukturelle niveau. Det er en enkel rørledning, der starter fra proteinrensning til røntgendatabehandling. Crystallogenesis, datasæt indeksering, og molekylær udskiftning er blevet understreget gennem et studie, TmPep1050H60A H307A variant.

Tags

Biokemi protein oligomerisering M42 aminopeptidase oligomeric tilstand overgang metalloenzym proteinrensning protein krystallisering X-ray krystallografi databehandling molekylær udskiftning
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter