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JoVE Journal Cancer Research
Ensaios para validação de inibidores de acetiltransferase histone
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Ensaios para validação de inibidores de acetiltransferase histone

Article DOI: 10.3791/61289-v • 09:11 min • August 6th, 2020
August 6th, 2020 •
1, 1
1Department of Anatomy and Cell Biology, and UF Health Cancer Center, University of Florida College of Medicine

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Inibidores de acetiltransferases de histona (HATs, também conhecidos como acetiltransferases de lisina), como CBP/p300, são potenciais terapêuticos para o tratamento do câncer. No entanto, são necessários métodos rigorosos para validar esses inibidores. Três métodos in vitro para validação incluem ensaios HAT com acetiltransferases recombinantes, imunoblotting para acetilação de histona na cultura celular, e ChIP-qPCR.

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Pesquisa do Câncer Questão 162 acetiltransferases de lisina KATs CBP/p300 inibidores da histona acetiltransferase métodos de triagem acetilação de histona epigenética câncer regulação genética
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