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JoVE Journal
Cancer Research

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自动差分核染色测定,准确测定三元细胞毒性
 
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自动差分核染色测定,准确测定三元细胞毒性

Article DOI: 10.3791/61295-v 07:58 min May 12th, 2020
May 12th, 2020

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Summary

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该协议描述了一种快速、高通量、可靠、廉价和公正的检测方法,用于有效确定细胞的可行性。当细胞的线粒体受损时,这种测定特别有用,这干扰了其他检测。该测定使用自动计数细胞染色的两种核染料 - Hoechst 33342 和碘化丙二钠。

Tags

本月在 JoVE, 问题 159, 高通量筛查, 线粒体靶向化合物 (米托根), 细胞毒性测定, 白血病, 荧光显微镜, 自动细胞计数
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