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Journal
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Bioengineering
/
数百个
C.白化病
细胞生物原子力显微镜测量自动化
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Automation of Bio-Atomic Force Microscope Measurements on Hundreds of
C. albicans
Cells
Please note that all translations are automatically generated.
Click here for the English version.
数百个
C.白化病
细胞生物原子力显微镜测量自动化
DOI:
10.3791/61315-v
•
09:27 min
•
April 02, 2021
•
Childérick Severac*
1
,
Sergio Proa-Coronado*
1,2,3
,
Cécile Formosa-Dague
,
Adrian Martinez-Rivas
5
,
Etienne Dague
1
ITAV-CNRS
,
Université de Toulouse, CNRS, Toulouse, France
,
2
LAAS-CNRS
,
Université de Toulouse, CNRS, Toulouse, France
,
3
ENCB, Instituto Politécnico Nacional (IPN), Mexico City, Mexico
,
4
TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France
,
5
CIC, Instituto Politécnico Nacional (IPN), Mexico City, Mexico
Chapters
00:04
Introduction
01:03
Polydimethylsiloxane (PDMS) Stamp Preparation
02:06
Cell Immobilization
03:21
Atomic Force Microscopy (AFM) Setup
03:58
AFM Imaging
06:26
Data Analysis
07:45
Results: Representative Median Stiffness Analyses
08:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议旨在自动化数百个微生物细胞上的自动对焦M测量。首先,微生物固定在PDMS标记显微结构中,然后在数百个固定细胞上自动进行力光谱测量。
Tags
Atomic Force Microscope Automation
Biomedical Applications
Biomechanical Properties
Cell Populations
C. Albicans Cells
PDMS Stamp
Cell Suspension
Microstructures
Cell Trapping
AFM Measurements
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