Biology
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建立和维护侏罗育美国蟑螂 （佩里普拉内塔美洲）
Chapters
Summary May 28th, 2021
Please note that all translations are automatically generated.
Click here for the English version.
该协议用于建立和维持侏罗育美国蟑螂（佩里普拉内塔美洲）通过表面消毒鸡蛋的情况下（oothecae）孵化前。这些侏罗纪昆虫含有垂直传播的 布拉塔细菌 内生菌，但有斧头内脏。
Transcript
侏儒生化蟑螂生成协议有助于测试本地肠道微生物群落去除和更换对宿主健康和微生物组件组装的影响。该技术使用来自多个来源的方法，形成一个有凝聚力的，简化的工作流程，用于在不使用昂贵的专业实验室设备的情况下产生侏托生物蟑螂。考虑到这种侏罗万象协议中许多不同的运动部分，可视化有助于研究人员更容易地建立自己的小规模、低成本、长凳顶的侏罗万象设施。
为了方便收集适当数量的携带卵母兽进行计划实验的女性，使用钳子移动库存罐中含有雌性毛发的纸板管。如果纸板管除了雌性外还含有多种昆虫，则将管内物摇入一个额外的塑料容器中，用石油果冻环成，并鼓励目标昆虫单独爬回纸板管中。收集后，将每位受精打细打的女性转移到产房。
一旦雌性掉落了卵母，将雌性送回储物箱，然后用钳子从垃圾中取回卵母鸡。要清洁卵子，在含有三毫升硫酸钠的五毫升离心管中放置多达五个鸡蛋盒，并在卵石中旋转 10 秒。经过第二次洗涤，如刚刚证明的那样，使用细腻的任务擦拭轻轻擦洗每个ootheca的表面，以清除任何碎片，并将清洁的ootheca放入称重船。
灭菌前，每管填充两根1.5毫升离心机管，每管一毫升无菌水。还要在烟罩中的五毫升离心机管中加入 10 微升 32% 的乙酸汤溶液，加入 3.2 毫升双蒸馏水。盖和倒数次混合，并放置多达五个清洁的卵轮在新鲜准备的0.1%的渗透酸溶液5分钟，倒置管几次每60秒。
渗透酸是一种强氧化剂，对眼睛、皮肤和呼吸刺激物有刺激性，具有易燃性和腐蚀性。在处理和妥善处理废物时，始终佩戴适当的 PPE。在孵化结束时，使用无菌钳子和层压流罩将每个卵母管转移到自己的离心机管中，即无菌冲洗水。
在将水箱转移到第二组冲洗水管之前，倒置数次混合。第二次冲洗后，使用无菌钳将卵石转移到单独的 BHI 倾斜处。将倾斜放入消毒的二级容器中，将容器移入潮湿的 30 摄氏度孵化器中，持续四到五周，直到孵化。
定期检查倾斜每周一至两次真菌或细菌生长，直到第四周，此时应每天检查倾斜。当仙女开始孵化时，使用无菌钳子和层压流罩将消毒的老鼠颗粒转移到准备好的BHI烧瓶中。作为无菌检查，将烧瓶放在30摄氏度的孵化器中24小时，以确认缺乏污染物生长。
如果烧瓶保持无菌，将仙女从倾斜中摇出，让它们落入层压流罩中的烧瓶中。然后每周在层压流罩中用 300 微升无菌水为仙女浇水一次。当仙女粪便开始覆盖中层时，将仙女转移到一个新的BHI烧瓶中，里面装着消毒过的老鼠，如前所示，提前24小时添加。
怀孕的女性可以通过附着在后腹部的卵母识别。手术箱在绝育后平均孵化34天。在绝育失败的情况下，在孵化过程中，在卵母周围会出现生长，损害幼崽的侏罗育生物状态。
然后，可以通过测量昆虫的身体长度来跟踪仙女的生长速度。来自同质化仙女的16S核糖核酸的限量片段长度多态性可用于确认侏儒生物状态。侏罗育蟑螂可以接种合成或异种生物微生物群落。
虽然这个特殊的工作流程是新的，但以前使用侏儒蟑螂的研究已经深入了解了肠道微生物群的组装和微生物在塑造社会行为、肠道健康和昆虫宿主免疫力方面的作用。
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