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Neuroscience

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JoVE Journal Neuroscience
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En model af Epileptogenese i Rhinal Cortex-Hippocampus Organotypic Slice Cultures

Article DOI: 10.3791/61330-v • 10:05 min • March 18th, 2021
March 18th, 2021 •
1,2, *1,2, *1,2, 1,2
1Instituto de Farmacologia e Neurociências, Faculdade de Medicina da Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina da Universidade de Lisboa
* These authors contributed equally

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Her beskriver vi forberedelsen af rhinal cortex-hippocampus organotypic skiver. Under en gradvis og kontrolleret afsavn af serum skildrer disse skiver udviklende epileptiske-lignende begivenheder og kan betragtes som en ex vivo-model af epileptogenese. Dette system repræsenterer et glimrende værktøj til overvågning af dynamikken i spontan aktivitet samt til vurdering af udviklingen af neuroinflammatoriske træk i løbet af epileptogenese.

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Neurovidenskab Organotypic skiver epileptogenese propidium jod gliosis CD68 C3d
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