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Chemistry

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JoVE Journal Chemistry
Automatiseret prøve multiplexing ved hjælp af kombineret precursor isotop mærkning og isoobaric tagging (cPILOT)
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Automatiseret prøve multiplexing ved hjælp af kombineret precursor isotop mærkning og isoobaric tagging (cPILOT)

Article DOI: 10.3791/61342-v • 09:24 min • December 18th, 2020
December 18th, 2020 •
1, 1,2,3,4
1Department of Chemistry, Vanderbilt University, 2Vanderbilt Memory & Alzheimer's Center, Vanderbilt University Medical Center, 3Vanderbilt Brain Institute, Vanderbilt University Medical Center, 4Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center

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Kombineret prækursor isotop mærkning og isoobaric tagging (cPILOT) er en forbedret prøve multiplexing strategi, der er i stand til at øge antallet af prøver, der kan analyseres samtidig med tilgængelige isobaric tags. Inkorporering af en robotplatform har øget den eksperimentelle overførselshastighed, reproducerbarhed og kvantitative nøjagtighed betydeligt.

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Kemi High-throughput Proteomics Dimethylation cPILOT robot væske handler Automation Isobaric Tags
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