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Journal / Biochemistry / Spektrofotometrisk screening for potensielle…
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Biochemistry
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JoVE Journal Biochemistry
Spektrofotometrisk screening for potensielle hemmere av cytosolisk glutation S-Transferases
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Spektrofotometrisk screening for potensielle hemmere av cytosolisk glutation S-Transferases

Article DOI: 10.3791/61347-v • 14:57 min • October 10th, 2020
October 10th, 2020 •
1,2, *1,3, *1,3
1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva
* These authors contributed equally

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Glutation S-transferases (GSTs) er avgiftning enzymer involvert i metabolismen av mange kjemoterapeutiske legemidler. Overuttrykk av GSTs er korrelert med kreft kjemoterapi resistens. En måte å motvirke denne fenotypen på er å bruke inhibitorer. Denne protokollen beskriver en metode ved hjelp av en spektrofotometrisk analyse for å screene for potensielle GST-hemmere.

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Biokjemi utgave 164 Glutation S-transferases GST-hemmere enzymanalyse hemmingsanalyse IC50 Ki
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