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Journal / Immunology and Infection / Digestione di interi occhi di topo per l'analisi…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestione di interi occhi di topo per l'analisi citometrica a flusso multi-parametro dei fagociti mononucleari
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Digestione di interi occhi di topo per l'analisi citometrica a flusso multi-parametro dei fagociti mononucleari

Article DOI: 10.3791/61348-v • 09:58 min • June 17th, 2020
June 17th, 2020 •
1, *2, *1
1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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Questo protocollo fornisce un metodo per digerire interi occhi in una singola sospensione cellulare ai fini dell'analisi citometrica del flusso multi-parametro al fine di identificare specifiche popolazioni fagocitiche mononucleari oculari, inclusi monociti, microglia, macrofagi e cellule dendritiche.

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Immunologia e Infezione Numero 160 occhio citometria del flusso macrofago microglia monocita mieloide
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