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Immunology and Infection

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JoVE Journal Immunology and Infection
Digestión de ojos enteros del ratón para el análisis citométrico de flujo multiparamétrico de fagocitos mononucleares
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Digestión de ojos enteros del ratón para el análisis citométrico de flujo multiparamétrico de fagocitos mononucleares

Article DOI: 10.3791/61348-v • 09:58 min • June 17th, 2020
June 17th, 2020 •
1, *2, *1
1Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, 2Department of Medicine, Division of Rheumatology, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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Este protocolo proporciona un método para digerir los ojos enteros en una sola suspensión celular con el propósito de análisis citométrico de flujo multiparamétrico con el fin de identificar poblaciones fagocíticas mononucleares oculares específicas, incluyendo monocitos, microglia, macrófagos y células dendríticas.

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Inmunología e infección Número 160 ojo citometría de flujo macrófago microglia monocito mieloide
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