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Immunology and Infection

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JoVE Journal Immunology and Infection
Karakterisering van immuuncellen en pro-inflammatoire mediatoren in de pulmonale omgeving
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Karakterisering van immuuncellen en pro-inflammatoire mediatoren in de pulmonale omgeving

Article DOI: 10.3791/61359-v • 09:00 min • June 24th, 2020
June 24th, 2020 •
*1, *1, 1, 1,2,3
1Department of Microbiology, Immunology, and Cell Biology, West Virginia University, 2Department of Neuroscience, West Virginia University, 3Rockefeller Neuroscience Institute, West Virginia University
* These authors contributed equally

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Dit protocol beschrijft het gebruik van flowcytometrie om de veranderingen in de samenstelling van immuuncellen, het cytokineprofiel en het chemokineprofiel in de pulmonale omgeving te identificeren na voorbijgaande occlusie van de middelste hersenslagader, een muizenmodel van ischemische beroerte.

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Immunologie en infectie pulmonale immuniteit immuuncelprofilering cytokines chemokines flowcytometrie multiplex bead arrays ischemische beroerte
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