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Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

Article DOI: 10.3791/61363-v • 06:10 min • May 13th, 2020
May 13th, 2020 •
1, 1
1Department of Biological Sciences, Lehigh University

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The goal of this procedure is to dissect the dorsal longitudinal muscle (DLM) tissue to assess the structural integrity of DLM neuromuscular junctions (NMJs) in neurodegenerative disease models using Drosophila melanogaster.

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Visualizing Synaptic Degeneration Adult Drosophila Neurodegeneration Protocol Assessing Synaptic Integrity Age Dependent Neurodegenerative Diseases Tissues Structural Analysis Functional Analysis Neuronal Tissue Muscle Tissue Neuromuscular Junctions Investigate Difficult To Work With Dissection Anesthesia Flies Petri Dish Ethanol Paint Brush Forceps Wings Silicone Elastomer Coated Dissection Dish PBS Dissecting Microscope Blunt Dumont Forceps Incision Ventral Side Of The Cuticle
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