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Immunology and Infection

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JoVE Journal Immunology and Infection
Live-Cell Forward Genetisk tilnærming til å identifisere og isolere utviklingsmutanter i Chlamydia trachomatis
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Live-Cell Forward Genetisk tilnærming til å identifisere og isolere utviklingsmutanter i Chlamydia trachomatis

Article DOI: 10.3791/61365-v • 10:32 min • June 10th, 2020
June 10th, 2020 •
1, 1, 1
1Department of Biological Sciences, University of Idaho

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Denne protokollen benytter fluorescerende promotor-reportere, live-celle mikroskopi, og individuell inkludering utvinning i en rettet fremover genetisk tilnærming for å identifisere og isolere utviklingsmutanter av Chlamydia trachomatis.

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Immunologi og infeksjon utgave 160 Fremgenetikk kjemisk mutagenese klamydiale utvikling live-celle mikroskopi automatisert mikroskopi fluorescerende reportere
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