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Journal / Genetics / CRISPR-Cas9-medieret Genom Redigering i…
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Genetics

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JoVE Journal Genetics
CRISPR-Cas9-medieret Genom Redigering i filamentøse Ascomycete Huntiella omanensis
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CRISPR-Cas9-medieret Genom Redigering i filamentøse Ascomycete Huntiella omanensis

Article DOI: 10.3791/61367-v • 07:25 min • June 9th, 2020
June 9th, 2020 •
1,2, 1,2
1Department of Biochemistry, Genetics & Microbiology, University of Pretoria, 2Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria

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CRISPR-Cas9 genomredigeringssystemet er en brugervenlig genomeditor, der er blevet brugt i modelarter og ikke-modelarter. Her præsenterer vi en protein-baseret version af dette system, der blev brugt til at indføre en for tidlig stop codon i et parringsgen af en ikke-model glødetråd ascomycete svamp.

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Genetik Genom redigering CRISPR-Cas9 RNP Transformationer Seksuel reproduktion Svampe Huntiella omanensis
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