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JoVE Journal
Neuroscience

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Kvantitative metoder til scoring in vivo Neuronal Aggregat og Organelle Extrusion i Store Exopher Vesikler i C. elegans
 

Kvantitative metoder til scoring in vivo Neuronal Aggregat og Organelle Extrusion i Store Exopher Vesikler i C. elegans

Article doi: 10.3791/61368
September 18th, 2020 Usage Statistics

Summary September 18th, 2020

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Denne protokol beskriver metoder til påvisning og kvantisering af store aggregerede og/eller organelle ekstruderinger (~4 μm), der produceres af C. elegans celler i form af membranbundne exfoner. Vi beskriver stammer, vækstbetingelser, scoringskriterier, timing og mikroskopi overvejelser, der er nødvendige for at lette dissektion af denne debris udvisning mekanisme.

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