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Anvendelse af Laser Microdissection at afdække regionale Transcriptomics i Human Nyre Tissue
 
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Anvendelse af Laser Microdissection at afdække regionale Transcriptomics i Human Nyre Tissue

Article DOI: 10.3791/61371
June 9th, 2020

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Summary June 9th, 2020

Please note that all translations are automatically generated.

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Vi beskriver en protokol for laser mikrodissementering af undersegmenter af den menneskelige nyre, herunder glomerulus, proksimal tubule, tykt stigende lem, opsamlingskanal og interstitium. RNA'et isoleres derefter fra de opnåede rum, og der udføres RNA-sekventering for at bestemme ændringer i transskriptionssignaturen inden for hvert undersegment.

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