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Journal / Genetics / 胚胎微注射技术，用于在库莱克斯五角星高效位点特异性突变体…
JoVE Journal
Genetics

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JoVE Journal Genetics
胚胎微注射技术，用于在库莱克斯五角星高效位点特异性突变体
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Journal (Genetics)
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
Journal (Genetics)
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
 
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胚胎微注射技术，用于在库莱克斯五角星高效位点特异性突变体

Article DOI: 10.3791/61375-v • 05:59 min • May 24th, 2020
May 24th, 2020 •
1, 1, 1, 2, 1,3
1Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology and Plant Patholog, Auburn University, 3Tata Institute for Genetics and Society, University of California, San Diego

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该协议描述了 Culex五角星胚胎的 微注射程序，这些胚胎通过CRISPR/Cas9基因编辑工具进行优化。该技术可以有效地生成特定地点、遗传的生殖系突变，可用于在这种研究不足的疾病载体中构建遗传技术。

Tags

遗传学， 第 159 期， 库莱克斯五角星， CRISPR/Cas9 基因组编辑， 胚胎对齐， 胚胎微注射， 基因组修饰， 生殖系突变
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