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Journal / Genetics / Embryo micro-injection technieken voor efficiënte …
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Genetics

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JoVE Journal Genetics
Embryo micro-injection technieken voor efficiënte site-specifieke mutagenese in Culex quinquefasciatus
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Embryo micro-injection technieken voor efficiënte site-specifieke mutagenese in Culex quinquefasciatus

Article DOI: 10.3791/61375-v • 05:59 min • May 24th, 2020
May 24th, 2020 •
1, 1, 1, 2, 1,3
1Section of Cell and Developmental Biology, University of California, San Diego, 2Department of Entomology and Plant Patholog, Auburn University, 3Tata Institute for Genetics and Society, University of California, San Diego

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Dit protocol beschrijft micro-injectieprocedures voor Culex quinquefasciatus embryo's die zijn geoptimaliseerd om te werken met CRISPR/Cas9 genbewerkingstools. Deze techniek kan efficiënt site-specifieke, erfelijke, kiembaanmutaties genereren die kunnen worden gebruikt voor het bouwen van genetische technologieën in deze onderbelichte ziektevector.

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Genetica Culex quinquefasciatus CRISPR/Cas9 genoombewerking embryo alignment embryo microinjection genoommodificatie germline mutagenese
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