JoVE Journal
Neuroscience
Neuroscience
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Summary
Please note that all translations are automatically generated.
这是一个急性切片制备从成人和老化的小鼠海马,利用转心灌注和切片切割与冰冷的NMDG-aCSF,以减少对组织的低氧损伤。生成的切片在几个小时内保持健康,并适用于长期的贴片夹和现场记录。