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Minimizando a hipóxia em fatias hipocampais de camundongos adultos e envelhecidos
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Minimizando a hipóxia em fatias hipocampais de camundongos adultos e envelhecidos

Article DOI: 10.3791/61377-v • 08:58 min • July 2nd, 2020
July 2nd, 2020 •
1
1Department of Biology, Neurobiology, and Bio-X, Stanford University

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Este é um protocolo para preparação aguda de fatias de hipocampi de camundongos adultos e envelhecidos que se aproveita da perfusão transcárrica e corte de fatias com NMDG-aCSF gelado para reduzir danos hipóxicos ao tecido. As fatias resultantes permanecem saudáveis durante muitas horas, e são adequadas para grampos de remendo de longo prazo e gravações de campo.

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Neurociência Edição 161 fatias hipocampais adulto envelhecimento hipóxia ATP NMDG CA1 patch-clamp registros de campo
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